Some problems in relation to the structures of insulin derivatives.
نویسندگان
چکیده
butyl ester), was done in the conventional way. Using this combination, [ ~ A l a ~ ~ ) IDHI was obtained in crystalline form and gave about 50% of the insulin activity in the mouseconvulsion assay. Insulins from different species are analogues formed in the course of evolution and are very useful in structure-and-function studies. We have worked out a simple method for isolating these analogues. Insulins of chicken, snake (Cao et al., 1980) and goose (Xu et al., 1982) have been isolated in crystalline form. The crystalline shape of snake insulin is very unusual, it is dodecahedral instead of rhombohedral. Snake 8-granules were studied by Watari & Hotta (1976), by high-voltage electron microscopy, and by Lange (1980), by electron microscopy and electron diffraction. They also found dodecahedral microcrystals and two possible space groups; P4,32 and P2,3 were proposed by Lange (1980). The space group of snake insulin we studied is P4,32 with 24 general positions, the unit cell is cubic with a equal to 0.673 1 nm (67.3 1 A) and contains 24 molecules in the form of four hexamers (Liang et al., 1983). In each hexamer, three 2-fold axes are perpendicular to a 3-fold axis, and each asymmetric unit contains only one molecule of insulin. Goose insulin was also crystallized and has been sequenced (Xu et al., 1982). It is similar to snake insulin in crystalline shape (Fig. 4) and identical with duck insulin in sequence. In the course of isolating insulin, goose C-peptide and pancreatic polypeptide were obtained as by-products and these have also been partially sequenced.
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عنوان ژورنال:
- Biochemical Society transactions
دوره 11 4 شماره
صفحات -
تاریخ انتشار 1983